murine macrophage cell line raw264 7  (ATCC)

Journal: Frontiers in Immunology

Article Title: Development and experimental validation of an osteoporosis diagnosis model based on disulfidoptosis-related genes and immune infiltration analysis

doi: 10.3389/fimmu.2026.1834059

Figure Lengend Snippet: Experimental validation of key gene expression in a RANKL-induced osteoclast differentiation model. (A) Representative micrographs showing morphological changes of RAW264.7 cells after 6 days of RANKL (50 ng/mL) induction. Scale bar = 50 μm. (B) mRNA expression levels of SOAT2, TUBA8, OR10H1, and KCNF1 detected by qPCR. GAPDH was used as an internal control. The mRNA level of FOLR3 was not determined (N.D.). (C) Quantitative densitometric analysis of western blot results. (D) Representative western blot images showing protein expression levels of FOLR3, KCNF1, OR10H1, SOAT2, and TUBA8. GAPDH was used as a loading control (n=3).*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.

Article Snippet: The murine RAW264.7 cells (Procell, Wuhan, China) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Pricella, PM150210, Wuhan, China) supplemented with 10% fetal bovine serum (FBS; Pricella, 164210, Wuhan, China) and 1% penicillin/streptomycin (Solarbio, P1400, Beijing, China).

Techniques: Biomarker Discovery, Gene Expression, Expressing, Control, Western Blot

Journal: Transboundary and Emerging Diseases

Article Title: Toxoplasma gondii KCR is a Noncanonical Modulator of CSF2 Signaling that Targets the CSF2Rα–JAK2/STAT5 Axis

doi: 10.1155/tbed/8426765

Figure Lengend Snippet: Activation of the JAK2/STAT5 pathway by KCR in macrophages. (A, C) Western blot analysis of phosphorylated JAK2 (p‐JAK2) and STAT5 (p‐STAT5) in RAW264.7 mouse macrophages stimulated with murine CSF2. (B, D) Densitometric quantification of p‐JAK2 and p‐STAT5 levels from (A) and (C), respectively. (E, G) Western blot analysis of p‐JAK2 and p‐STAT5 in RAW264.7 macrophages stimulated with KCR. (F, H) Densitometric quantification of p‐JAK2 and p‐STAT5 levels from (E) and (G), respectively. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001.

Article Snippet: Human foreskin fibroblasts (HFFs), HEK 293T cells, and murine macrophage RAW264.7 cells were purchased from the American Type Culture Collection and are currently maintained in our laboratory.

Techniques: Activation Assay, Western Blot